2QK9
Human RNase H catalytic domain mutant D210N in complex with 18-mer RNA/DNA hybrid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | 5'-R(*AP*GP*UP*GP*CP*GP*AP*CP*AP*CP*CP*UP*GP*AP*UP*UP*CP*C)-3' | polymer | 18 | 5708.4 | 1 | |||
| 2 | B (C) | 5'-D(*GP*GP*AP*AP*TP*CP*AP*GP*GP*TP*GP*TP*CP*GP*CP*AP*CP*T)-3' | polymer | 18 | 5556.6 | 1 | |||
| 3 | C (A) | Ribonuclease H1 | polymer | 154 | 17104.3 | 1 | UniProt (O60930) Pfam (PF00075) | Homo sapiens (human) | Hs-RNase HC; RNase H1; Ribonuclease H type II |
| 4 | D, E, F, J, K... (B, C, A) | SULFATE ION | non-polymer | 96.1 | 7 | Chemie (SO4) | |||
| 5 | G (C) | HEXANE-1,6-DIAMINE | non-polymer | 116.2 | 1 | Chemie (16D) | |||
| 6 | H (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 7 | I (A) | CITRATE ANION | non-polymer | 189.1 | 1 | Chemie (FLC) | |||
| 8 | N, O (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 9 | P, Q, R (B, C, A) | water | water | 18.0 | 109 | Chemie (HOH) |
Sequence modifications
A: 136 - 286 (UniProt: O60930)
| PDB | External Database | Details |
|---|---|---|
| Gly 133 | - | expression tag |
| Ser 134 | - | expression tag |
| His 135 | - | expression tag |
| Asn 210 | Asp 210 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 28369.3 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1184.9 | |
| All* | Total formula weight | 29554.2 |
