2QK9
Human RNase H catalytic domain mutant D210N in complex with 18-mer RNA/DNA hybrid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 158.578, 158.578, 142.055 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.550 |
| Rwork | 0.190 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Original low resolution strcture solved with the same crystal and SeMet SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.074 | 0.456 |
| Number of reflections | 21164 | |
| <I/σ(I)> | 38.7 | 4.1 |
| Completeness [%] | 94.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 1.5 M ammonium sulfate, 0.1 M Na citrate (pH 5.6) 20mM ATP or 3% 1,6-diaminohexane, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | sodium citrate | ||
| 3 | 1 | 1 | 1,6-diaminohexane | ||
| 4 | 1 | 2 | (NH4)2SO4 | ||
| 5 | 1 | 2 | sodium citrate | ||
| 6 | 1 | 2 | 1,6-diaminohexane |
