2P22

Structure of the Yeast ESCRT-I Heterotetramer Core

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease	polymer	174	19903.4	1	UniProt (P25604) Pfam (PF09454) In PDB	Saccharomyces cerevisiae (baker's yeast)	Vacuolar protein sorting-associated protein 23
2	B	Vacuolar protein sorting-associated protein 28	polymer	118	13755.3	1	UniProt (Q02767) Pfam (PF03997) In PDB	Saccharomyces cerevisiae (baker's yeast)
3	C	Protein SRN2	polymer	192	22598.5	1	UniProt (Q99176) Pfam (PF07200) In PDB	Saccharomyces cerevisiae (baker's yeast)	Vacuolar protein sorting-associated protein 37
4	D	Hypothetical 12.0 kDa protein in ADE3-SER2 intergenic region	polymer	79	9661.0	1	UniProt (P42939) Pfam (PF09452) In PDB	Saccharomyces cerevisiae (baker's yeast)
5	C, D	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
6		water	water		18.0	22	Chemie (HOH)

Sequence modifications

A: 215 - 385 (UniProt: P25604)

PDB	External Database	Details
Gly 212	-	cloning artifact
Ala 213	-	cloning artifact
Met 214	-	cloning artifact

B: 1 - 118 (UniProt: Q02767)

PDB	External Database	Details
Ala 101	Cys 101	engineered mutation

C: 22 - 213 (UniProt: Q99176)

PDB	External Database	Details
Ala 123	Cys 123	engineered mutation

D: 4 - 81 (UniProt: P42939)

PDB	External Database	Details
Met 3	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	65918.2
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	66302.5

*Water molecules are not included.