2OKC

Crystal structure of Type I restriction enzyme StySJI M protein (NP_813429.1) from Bacteroides thetaiotaomicron VPI-5482 at 2.20 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Type I restriction enzyme StySJI M protein	polymer	445	50657.6	2	UniProt (Q89Z59) Pfam (PF12161) Pfam (PF02384)	Bacteroides thetaiotaomicron VPI-5482
2	C, G (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (58766358) PubChem (73897310) PubChem (9865603) PubChem (87224684) PubChem (12312798) PubChem (131697735) PubChem (145712080) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094)
3	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
4	E, H (A, B)	ISOPROPYL ALCOHOL	non-polymer		60.1	2	Chemie (IPA)
5	F (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
6	I, J (A, B)	water	water		18.0	348	Chemie (HOH)

Sequence modifications

A, B: 1 - 444 (UniProt: Q89Z59)

PDB	External Database	Details
Gly 0	-	expression tag
Mse 1	Met 1	modified residue
Mse 44	Met 44	modified residue
Mse 51	Met 51	modified residue
Mse 116	Met 116	modified residue
Mse 125	Met 125	modified residue
Mse 162	Met 162	modified residue
Mse 170	Met 170	modified residue
Mse 192	Met 192	modified residue
Mse 225	Met 225	modified residue
Mse 292	Met 292	modified residue
Mse 293	Met 293	modified residue
Mse 295	Met 295	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	101315.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1044.6
All*	Total formula weight	102359.8

*Water molecules are not included.