2NS1
Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ammonia channel | polymer | 412 | 42952.3 | 1 | UniProt (P69681) Pfam (PF00909) | Escherichia coli | Ammonia transporter |
| 2 | B (B) | Nitrogen regulatory protein P-II 2 | polymer | 116 | 12558.5 | 1 | UniProt (P0AC55) Pfam (PF00543) | Escherichia coli | |
| 3 | C, D, E, F, G... (A) | octyl beta-D-glucopyranoside | non-polymer | 292.4 | 8 | Chemie (BOG) | |||
| 4 | K, L (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) | |||
| 5 | M (B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 6 | N, O (A, B) | water | water | 18.0 | 498 | Chemie (HOH) |
Sequence modifications
A: 1 - 406 (UniProt: P69681)
B: 1 - 112 (UniProt: P0AC55)
| PDB | External Database | Details |
|---|---|---|
| Gly 407 | - | cloning artifact |
| Thr 408 | - | cloning artifact |
| Leu 409 | - | cloning artifact |
| Val 410 | - | cloning artifact |
| Pro 411 | - | cloning artifact |
| Arg 412 | - | cloning artifact |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | cloning artifact |
| Pro -2 | - | cloning artifact |
| Gly -1 | - | cloning artifact |
| Ser 0 | - | cloning artifact |
| Phe 51 | Tyr 51 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 55510.8 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 3010.4 | |
| All* | Total formula weight | 58521.2 |
