2NS1

Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-24
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1159
Spacegroup name	H 3 2
Unit cell lengths	102.032, 102.032, 363.849
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.070 - 1.962
R-factor	0.16435
Rwork	0.162
R-free	0.19838
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1U7G and PDB ENTRY 2GNK
RMSD bond length	0.012
RMSD bond angle	1.484
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.070	2.030
High resolution limit [Å]	1.962	1.962
Number of reflections	52357
<I/σ(I)>	41.2	2.1
Completeness [%]	98.9	93.4
Redundancy	7.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20% PEG 550MME, 0.12M TRIS(HYDROXYMETHYL)AMINOMETHANE, 2mM ADENOSINE-5'-TRIPHOSPHATE, 25mM AMONIUM SULFATE, 0.04M B-OCTYLGLUCOSIDE, 2mM DITHIOTHREITOL, 0.05M SODIUM CHLORIDE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K