2NS1
Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1159 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 102.032, 102.032, 363.849 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.070 - 1.962 |
| R-factor | 0.16435 |
| Rwork | 0.162 |
| R-free | 0.19838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1U7G and PDB ENTRY 2GNK |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.070 | 2.030 |
| High resolution limit [Å] | 1.962 | 1.962 |
| Number of reflections | 52357 | |
| <I/σ(I)> | 41.2 | 2.1 |
| Completeness [%] | 98.9 | 93.4 |
| Redundancy | 7.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG 550MME, 0.12M TRIS(HYDROXYMETHYL)AMINOMETHANE, 2mM ADENOSINE-5'-TRIPHOSPHATE, 25mM AMONIUM SULFATE, 0.04M B-OCTYLGLUCOSIDE, 2mM DITHIOTHREITOL, 0.05M SODIUM CHLORIDE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
