2NS1
Crystal structure of the e. coli ammonia channel AMTB complexed with the signal transduction protein GLNK
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0008519 | molecular_function | ammonium channel activity |
| A | 0015670 | biological_process | carbon dioxide transport |
| A | 0016020 | cellular_component | membrane |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0072488 | biological_process | ammonium transmembrane transport |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006808 | biological_process | regulation of nitrogen utilization |
| B | 0030234 | molecular_function | enzyme regulator activity |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0045848 | biological_process | positive regulation of nitrogen utilization |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 236 |
| Details | Transmembrane: {"description":"Helical","evidences":[{"source":"PubMed","id":"15361618","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15563598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17040913","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 48 |
| Details | Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"15361618","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15563598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17040913","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 62 |
| Details | Topological domain: {"description":"Periplasmic","evidences":[{"source":"PubMed","id":"15361618","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15563598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17040913","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 28 |
| Details | Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"15361618","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15563598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15919996","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17040913","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"15361618","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Site: {"description":"Important for the deprotonation of the ammonium cation","evidences":[{"source":"PubMed","id":"19278252","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Site: {"description":"Twin-His motif. Important for optimum substrate conductance","evidences":[{"source":"PubMed","id":"17040913","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"23667517","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"32662768","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Site: {"description":"Important for optimum substrate conductance","evidences":[{"source":"PubMed","id":"18362341","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"17220269","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2NUU","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"9733647","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2GNK","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"17190799","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17220269","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2NS1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2NUU","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"O-UMP-tyrosine","evidences":[{"source":"PROSITE-ProRule","id":"PRU00675","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
