2MUT

Solution structure of the F231L mutant ERCC1-XPF dimerization region

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA excision repair protein ERCC-1	polymer	96	10985.7	1	UniProt (P07992) Pfam (PF00633) In PDB	Homo sapiens (human)
2	B	DNA repair endonuclease XPF	polymer	84	9230.5	1	UniProt (Q92889) In PDB	Homo sapiens (human)	DNA excision repair protein ERCC-4, DNA repair protein complementing XP-F cells, Xeroderma pigmentosum group F-complementing protein

Sequence modifications

A: 220 - 297 (UniProt: P07992)

PDB	External Database	Details
Arg 212	-	expression tag
Ile 213	-	expression tag
Arg 214	-	expression tag
Arg 215	-	expression tag
Arg 216	-	expression tag
Tyr 217	-	expression tag
Asn 218	-	expression tag
Met 219	-	expression tag
Leu 231	Phe 231	engineered mutation
Gly 298	-	expression tag
Gly 299	-	expression tag
Leu 300	-	expression tag
Glu 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag

B: 823 - 905 (UniProt: Q92889)

PDB	External Database	Details
Met 822	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	20216.2
All*	Total formula weight	20216.2

*Water molecules are not included.