2MSE

NMR data-driven model of GTPase KRas-GNP:ARafRBD complex tethered to a lipid-bilayer nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Apolipoprotein A-I	polymer	200	23234.3	2	UniProt (P02647) Pfam (PF01442)	Homo sapiens (Human)	ApoA-I,Apolipoprotein A1
2	C (B)	GTPase KRas	polymer	187	21260.3	1	UniProt (P01116) Pfam (PF00071)	Homo sapiens (Human)	K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras
3	D (D)	Serine/threonine-protein kinase A-Raf	polymer	73	8133.5	1	UniProt (P10398) Pfam (PF02196)	Homo sapiens (Human)	Proto-oncogene A-Raf,Proto-oncogene A-Raf-1,Proto-oncogene Pks
4	AA, AB, BA, BB, CA... (A)	1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		787.1	64	Chemie (PCW)
5	AC, BC, CC, DC, EC... (A)	O-[(S)-({(2R)-2,3-bis[(9Z)-octadec-9-enoyloxy]propyl}oxy)(hydroxy)phosphoryl]-L-serine	non-polymer		788.0	16	Chemie (17F) PubChem (16219821) PubChem (57369815) PubChem (58556791) PubChem (125750) PubChem (3451126) PubChem (5830256) PubChem (6438639) PubChem (124226306) PubChem (99650225) PubChem (9547077) PubChem (9547078) PubChem (122607770) PubChem (9898005) PubChem (124629033) PubChem (145954209) PubChem (15409351)
6	GC (B)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	1	Chemie (GNP)
7	HC (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

A, C: 201 - 398 (UniProt: P02647)

PDB	External Database	Details
Gly 199	-	expression tag
Pro 200	-	expression tag

B: 1 - 185 (UniProt: P01116)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	75862.3
Non-Polymers*	Number of molecules	82
	Total formula weight	63530.9
All*	Total formula weight	139393.3

*Water molecules are not included.