2J6E

Crystal Structure of an Autoimmune Complex between a Human IgM Rheumatoid Factor and IgG1 Fc reveals a Novel Fc Epitope and Evidence for Affinity Maturation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	IG GAMMA-1 CHAIN C REGION	polymer	232	26140.6	2	UniProt (P01857) Pfam (PF07654)	HOMO SAPIENS (HUMAN)	IGG1
2	C, D (H, I)	IGM	polymer	231	24831.9	2		HOMO SAPIENS (HUMAN)	FAB HEAVY CHAIN
3	E, F (L, M)	IGM	polymer	234	24757.7	2		HOMO SAPIENS (HUMAN)	FAB LIGHT CHAIN
4	G, H (C, D)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-beta-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1625.5	2	In PDB GlyTouCan (G19282BU)
5	I (A)	CADMIUM ION	non-polymer		112.4	1	Chemie (CD)
6	J, Q (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	K (A)	CACODYLATE ION	non-polymer		137.0	1	Chemie (CAC)
8	L, M, N, R, S... (A, B, M)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	6	Chemie (MPD)
9	O, P, T, U (A, B, H)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
10	AA, BA, W, X, Y... (L, M, A, B, H...)	water	water		18.0	84	Chemie (HOH)

Sequence modifications

A, B: 216 - 447 (UniProt: P01857)

PDB	External Database	Details
Glu 270	Asp 153	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	151460.3
Branched	Number of molecules	2
	Total formula weight	3251.0
Non-Polymers*	Number of molecules	14
	Total formula weight	1325.4
All*	Total formula weight	156036.7

*Water molecules are not included.