2J6E
Crystal Structure of an Autoimmune Complex between a Human IgM Rheumatoid Factor and IgG1 Fc reveals a Novel Fc Epitope and Evidence for Affinity Maturation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 241.980, 75.610, 102.400 |
Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
Resolution | 25.000 - 3.000 |
R-factor | 0.2234 |
Rwork | 0.223 |
R-free | 0.28770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1DN2 2fb4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.369 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.080 | 0.420 |
Number of reflections | 36545 | |
<I/σ(I)> | 14.1 | 3.2 |
Completeness [%] | 97.9 | 93.8 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 10% MPEG 5000, 3 MM ZINC ACETATE, 3 MM CDCL2, 100 MM SODIUM CACODYLATE PH 6.5 |