2IU7
Site directed mutagenesis of key residues involved in the catalytic mechanism of Cyanase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | CYANATE HYDRATASE | polymer | 156 | 17051.8 | 10 | UniProt (P00816) Pfam (PF21291) Pfam (PF02560) | ESCHERICHIA COLI | CYANASE LYASE, CYANASE, CYANATE HYDROLASE |
| 2 | BA, K, Q, S, Y (G, A, C, D, F) | OXALATE ION | non-polymer | 88.0 | 5 | Chemie (OXL) | |||
| 3 | AA, CA, DA, EA, FA... (F, G, H, I, J...) | SULFATE ION | non-polymer | 96.1 | 21 | Chemie (SO4) | |||
| 4 | KA, LA, MA, NA, OA... (A, B, C, D, E...) | water | water | 18.0 | 3157 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H, I, J: 1 - 156 (UniProt: P00816)
| PDB | External Database | Details |
|---|---|---|
| Phe 95 | Tyr 95 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 170517.9 | |
| Non-Polymers* | Number of molecules | 26 |
| Total formula weight | 2457.4 | |
| All* | Total formula weight | 172975.3 |
