2IU7
Site directed mutagenesis of key residues involved in the catalytic mechanism of Cyanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | CUSTOM |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.514, 82.444, 84.498 |
| Unit cell angles | 61.90, 72.56, 69.59 |
Refinement procedure
| Resolution | 76.250 - 1.910 |
| R-factor | 0.134 |
| Rwork | 0.131 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dw9 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.647 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.030 | 0.100 |
| Number of reflections | 1 | |
| <I/σ(I)> | 25.4 | 7.3 |
| Completeness [%] | 96.8 | 91.2 |
| Redundancy | 2.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | CRYSTALS WERE GROWN BY THE SITTING DROP METHOD OF VAPOUR DIFFUSION FROM 50% AMMONIUM SULPHATE SOLUTIONS BUFFERED WITH 50MM NAKPO4, PH = 7.3, AND IN THE PRESENCE OF 50 MM TRIC/HCL, PH =7.3. |
