2IU7
Site directed mutagenesis of key residues involved in the catalytic mechanism of Cyanase
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0003677 | molecular_function | DNA binding |
A | 0008824 | molecular_function | cyanate hydratase activity |
A | 0009439 | biological_process | cyanate metabolic process |
A | 0009440 | biological_process | cyanate catabolic process |
A | 0016829 | molecular_function | lyase activity |
B | 0003677 | molecular_function | DNA binding |
B | 0008824 | molecular_function | cyanate hydratase activity |
B | 0009439 | biological_process | cyanate metabolic process |
B | 0009440 | biological_process | cyanate catabolic process |
B | 0016829 | molecular_function | lyase activity |
C | 0003677 | molecular_function | DNA binding |
C | 0008824 | molecular_function | cyanate hydratase activity |
C | 0009439 | biological_process | cyanate metabolic process |
C | 0009440 | biological_process | cyanate catabolic process |
C | 0016829 | molecular_function | lyase activity |
D | 0003677 | molecular_function | DNA binding |
D | 0008824 | molecular_function | cyanate hydratase activity |
D | 0009439 | biological_process | cyanate metabolic process |
D | 0009440 | biological_process | cyanate catabolic process |
D | 0016829 | molecular_function | lyase activity |
E | 0003677 | molecular_function | DNA binding |
E | 0008824 | molecular_function | cyanate hydratase activity |
E | 0009439 | biological_process | cyanate metabolic process |
E | 0009440 | biological_process | cyanate catabolic process |
E | 0016829 | molecular_function | lyase activity |
F | 0003677 | molecular_function | DNA binding |
F | 0008824 | molecular_function | cyanate hydratase activity |
F | 0009439 | biological_process | cyanate metabolic process |
F | 0009440 | biological_process | cyanate catabolic process |
F | 0016829 | molecular_function | lyase activity |
G | 0003677 | molecular_function | DNA binding |
G | 0008824 | molecular_function | cyanate hydratase activity |
G | 0009439 | biological_process | cyanate metabolic process |
G | 0009440 | biological_process | cyanate catabolic process |
G | 0016829 | molecular_function | lyase activity |
H | 0003677 | molecular_function | DNA binding |
H | 0008824 | molecular_function | cyanate hydratase activity |
H | 0009439 | biological_process | cyanate metabolic process |
H | 0009440 | biological_process | cyanate catabolic process |
H | 0016829 | molecular_function | lyase activity |
I | 0003677 | molecular_function | DNA binding |
I | 0008824 | molecular_function | cyanate hydratase activity |
I | 0009439 | biological_process | cyanate metabolic process |
I | 0009440 | biological_process | cyanate catabolic process |
I | 0016829 | molecular_function | lyase activity |
J | 0003677 | molecular_function | DNA binding |
J | 0008824 | molecular_function | cyanate hydratase activity |
J | 0009439 | biological_process | cyanate metabolic process |
J | 0009440 | biological_process | cyanate catabolic process |
J | 0016829 | molecular_function | lyase activity |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE OXL A1157 |
Chain | Residue |
A | ILE120 |
A | SER122 |
A | LEU151 |
D | ARG96 |
I | ARG96 |
J | ILE120 |
J | SER122 |
J | LEU151 |
site_id | AC2 |
Number of Residues | 3 |
Details | BINDING SITE FOR RESIDUE SO4 A1158 |
Chain | Residue |
A | GLU40 |
A | HOH2278 |
A | ALA39 |
site_id | AC3 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE SO4 A1159 |
Chain | Residue |
A | ARG87 |
A | HOH2201 |
A | HOH2279 |
A | HOH2280 |
B | ARG87 |
D | ARG87 |
J | ARG87 |
site_id | AC4 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE SO4 A1160 |
Chain | Residue |
A | ARG87 |
A | HOH2279 |
A | HOH2281 |
A | HOH2282 |
C | ARG87 |
I | ARG87 |
J | ARG87 |
site_id | AC5 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE SO4 B1157 |
Chain | Residue |
B | ALA39 |
B | GLU40 |
B | HOH2309 |
B | HOH2310 |
B | HOH2313 |
site_id | AC6 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE SO4 B1158 |
Chain | Residue |
B | ARG87 |
B | HOH2223 |
B | HOH2314 |
B | HOH2315 |
D | ARG87 |
E | ARG87 |
F | ARG87 |
site_id | AC7 |
Number of Residues | 9 |
Details | BINDING SITE FOR RESIDUE OXL C1157 |
Chain | Residue |
C | ILE120 |
C | SER122 |
C | ALA123 |
C | LEU151 |
H | ARG96 |
I | ILE120 |
I | SER122 |
I | LEU151 |
J | ARG96 |
site_id | AC8 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE SO4 C1158 |
Chain | Residue |
C | ALA39 |
C | GLU40 |
C | HOH2305 |
C | HOH2306 |
C | HOH2307 |
C | HOH2308 |
C | HOH2309 |
C | HOH2310 |
site_id | AC9 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE OXL D1157 |
Chain | Residue |
A | ARG96 |
B | ILE120 |
B | SER122 |
B | LEU151 |
D | ILE120 |
D | SER122 |
D | LEU151 |
F | ARG96 |
site_id | BC1 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE SO4 D1158 |
Chain | Residue |
D | ALA39 |
D | GLU40 |
D | HOH2299 |
D | HOH2300 |
D | HOH2301 |
D | HOH2302 |
D | HOH2303 |
site_id | BC2 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE SO4 D1159 |
Chain | Residue |
D | ALA33 |
D | ASP34 |
D | GLY35 |
D | THR36 |
D | GLY37 |
D | HOH2110 |
site_id | BC3 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE SO4 E1157 |
Chain | Residue |
E | ALA39 |
E | GLU40 |
E | HOH2290 |
E | HOH2291 |
E | HOH2292 |
E | HOH2293 |
site_id | BC4 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE SO4 E1158 |
Chain | Residue |
E | ARG87 |
E | HOH2294 |
E | HOH2295 |
F | ARG87 |
G | ARG87 |
G | HOH2212 |
H | ARG87 |
site_id | BC5 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE SO4 E1159 |
Chain | Residue |
E | ALA33 |
E | ASP34 |
E | GLY35 |
E | THR36 |
E | GLY37 |
site_id | BC6 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE OXL F1157 |
Chain | Residue |
F | LEU151 |
G | ARG96 |
B | ARG96 |
E | ILE120 |
E | SER122 |
E | LEU151 |
F | ILE120 |
F | SER122 |
site_id | BC7 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE SO4 F1158 |
Chain | Residue |
F | ALA39 |
F | GLU40 |
F | HOH2321 |
F | HOH2322 |
F | HOH2323 |
site_id | BC8 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE SO4 F1159 |
Chain | Residue |
F | ALA33 |
F | ASP34 |
F | GLY35 |
F | THR36 |
F | GLY37 |
F | HOH2325 |
F | HOH2326 |
site_id | BC9 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE OXL G1157 |
Chain | Residue |
C | ARG96 |
E | ARG96 |
G | ILE120 |
G | SER122 |
G | LEU151 |
H | ILE120 |
H | SER122 |
H | LEU151 |
site_id | CC1 |
Number of Residues | 4 |
Details | BINDING SITE FOR RESIDUE SO4 G1158 |
Chain | Residue |
G | ALA39 |
G | GLU40 |
G | HOH2288 |
G | HOH2290 |
site_id | CC2 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE SO4 H1157 |
Chain | Residue |
H | ALA39 |
H | GLU40 |
H | HOH2277 |
H | HOH2324 |
H | HOH2325 |
H | HOH2326 |
site_id | CC3 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE SO4 H1158 |
Chain | Residue |
H | ASP34 |
H | GLY35 |
H | THR36 |
H | GLY37 |
H | HOH2142 |
site_id | CC4 |
Number of Residues | 7 |
Details | BINDING SITE FOR RESIDUE SO4 I1157 |
Chain | Residue |
I | ALA39 |
I | GLU40 |
I | HOH2324 |
I | HOH2325 |
I | HOH2326 |
I | HOH2328 |
I | HOH2330 |
site_id | CC5 |
Number of Residues | 6 |
Details | BINDING SITE FOR RESIDUE SO4 I1158 |
Chain | Residue |
C | ARG87 |
C | HOH2219 |
G | ARG87 |
H | ARG87 |
I | ARG87 |
I | HOH2331 |
site_id | CC6 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE SO4 I1159 |
Chain | Residue |
I | ALA33 |
I | ASP34 |
I | GLY35 |
I | THR36 |
I | GLY37 |
site_id | CC7 |
Number of Residues | 5 |
Details | BINDING SITE FOR RESIDUE SO4 J1157 |
Chain | Residue |
J | ALA39 |
J | GLU40 |
J | HOH2370 |
J | HOH2371 |
J | HOH2372 |
site_id | CC8 |
Number of Residues | 8 |
Details | BINDING SITE FOR RESIDUE SO4 J1158 |
Chain | Residue |
J | ALA33 |
J | ASP34 |
J | GLY35 |
J | THR36 |
J | GLY37 |
J | HOH2149 |
J | HOH2373 |
J | HOH2374 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 30 |
Details | ACT_SITE: |
Chain | Residue | Details |
A | ARG96 | |
D | ARG96 | |
D | GLU99 | |
D | SER122 | |
E | ARG96 | |
E | GLU99 | |
E | SER122 | |
F | ARG96 | |
F | GLU99 | |
F | SER122 | |
G | ARG96 | |
A | GLU99 | |
G | GLU99 | |
G | SER122 | |
H | ARG96 | |
H | GLU99 | |
H | SER122 | |
I | ARG96 | |
I | GLU99 | |
I | SER122 | |
J | ARG96 | |
J | GLU99 | |
A | SER122 | |
J | SER122 | |
B | ARG96 | |
B | GLU99 | |
B | SER122 | |
C | ARG96 | |
C | GLU99 | |
C | SER122 |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
A | ARG96 | electrostatic stabiliser |
A | GLU99 | enhance reactivity |
A | SER122 | electrostatic stabiliser |
site_id | MCSA10 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
J | ARG96 | electrostatic stabiliser |
J | GLU99 | enhance reactivity |
J | SER122 | electrostatic stabiliser |
site_id | MCSA2 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
B | ARG96 | electrostatic stabiliser |
B | GLU99 | enhance reactivity |
B | SER122 | electrostatic stabiliser |
site_id | MCSA3 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
C | ARG96 | electrostatic stabiliser |
C | GLU99 | enhance reactivity |
C | SER122 | electrostatic stabiliser |
site_id | MCSA4 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
D | ARG96 | electrostatic stabiliser |
D | GLU99 | enhance reactivity |
D | SER122 | electrostatic stabiliser |
site_id | MCSA5 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
E | ARG96 | electrostatic stabiliser |
E | GLU99 | enhance reactivity |
E | SER122 | electrostatic stabiliser |
site_id | MCSA6 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
F | ARG96 | electrostatic stabiliser |
F | GLU99 | enhance reactivity |
F | SER122 | electrostatic stabiliser |
site_id | MCSA7 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
G | ARG96 | electrostatic stabiliser |
G | GLU99 | enhance reactivity |
G | SER122 | electrostatic stabiliser |
site_id | MCSA8 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
H | ARG96 | electrostatic stabiliser |
H | GLU99 | enhance reactivity |
H | SER122 | electrostatic stabiliser |
site_id | MCSA9 |
Number of Residues | 3 |
Details | M-CSA 845 |
Chain | Residue | Details |
I | ARG96 | electrostatic stabiliser |
I | GLU99 | enhance reactivity |
I | SER122 | electrostatic stabiliser |