2IU7
Site directed mutagenesis of key residues involved in the catalytic mechanism of Cyanase
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0008824 | molecular_function | cyanate hydratase activity |
| A | 0009440 | biological_process | cyanate catabolic process |
| A | 0016829 | molecular_function | lyase activity |
| B | 0003677 | molecular_function | DNA binding |
| B | 0008824 | molecular_function | cyanate hydratase activity |
| B | 0009440 | biological_process | cyanate catabolic process |
| B | 0016829 | molecular_function | lyase activity |
| C | 0003677 | molecular_function | DNA binding |
| C | 0008824 | molecular_function | cyanate hydratase activity |
| C | 0009440 | biological_process | cyanate catabolic process |
| C | 0016829 | molecular_function | lyase activity |
| D | 0003677 | molecular_function | DNA binding |
| D | 0008824 | molecular_function | cyanate hydratase activity |
| D | 0009440 | biological_process | cyanate catabolic process |
| D | 0016829 | molecular_function | lyase activity |
| E | 0003677 | molecular_function | DNA binding |
| E | 0008824 | molecular_function | cyanate hydratase activity |
| E | 0009440 | biological_process | cyanate catabolic process |
| E | 0016829 | molecular_function | lyase activity |
| F | 0003677 | molecular_function | DNA binding |
| F | 0008824 | molecular_function | cyanate hydratase activity |
| F | 0009440 | biological_process | cyanate catabolic process |
| F | 0016829 | molecular_function | lyase activity |
| G | 0003677 | molecular_function | DNA binding |
| G | 0008824 | molecular_function | cyanate hydratase activity |
| G | 0009440 | biological_process | cyanate catabolic process |
| G | 0016829 | molecular_function | lyase activity |
| H | 0003677 | molecular_function | DNA binding |
| H | 0008824 | molecular_function | cyanate hydratase activity |
| H | 0009440 | biological_process | cyanate catabolic process |
| H | 0016829 | molecular_function | lyase activity |
| I | 0003677 | molecular_function | DNA binding |
| I | 0008824 | molecular_function | cyanate hydratase activity |
| I | 0009440 | biological_process | cyanate catabolic process |
| I | 0016829 | molecular_function | lyase activity |
| J | 0003677 | molecular_function | DNA binding |
| J | 0008824 | molecular_function | cyanate hydratase activity |
| J | 0009440 | biological_process | cyanate catabolic process |
| J | 0016829 | molecular_function | lyase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE OXL A1157 |
| Chain | Residue |
| A | ILE120 |
| A | SER122 |
| A | LEU151 |
| D | ARG96 |
| I | ARG96 |
| J | ILE120 |
| J | SER122 |
| J | LEU151 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A1158 |
| Chain | Residue |
| A | GLU40 |
| A | HOH2278 |
| A | ALA39 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 A1159 |
| Chain | Residue |
| A | ARG87 |
| A | HOH2201 |
| A | HOH2279 |
| A | HOH2280 |
| B | ARG87 |
| D | ARG87 |
| J | ARG87 |
| site_id | AC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 A1160 |
| Chain | Residue |
| A | ARG87 |
| A | HOH2279 |
| A | HOH2281 |
| A | HOH2282 |
| C | ARG87 |
| I | ARG87 |
| J | ARG87 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 B1157 |
| Chain | Residue |
| B | ALA39 |
| B | GLU40 |
| B | HOH2309 |
| B | HOH2310 |
| B | HOH2313 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 B1158 |
| Chain | Residue |
| B | ARG87 |
| B | HOH2223 |
| B | HOH2314 |
| B | HOH2315 |
| D | ARG87 |
| E | ARG87 |
| F | ARG87 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE OXL C1157 |
| Chain | Residue |
| C | ILE120 |
| C | SER122 |
| C | ALA123 |
| C | LEU151 |
| H | ARG96 |
| I | ILE120 |
| I | SER122 |
| I | LEU151 |
| J | ARG96 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 C1158 |
| Chain | Residue |
| C | ALA39 |
| C | GLU40 |
| C | HOH2305 |
| C | HOH2306 |
| C | HOH2307 |
| C | HOH2308 |
| C | HOH2309 |
| C | HOH2310 |
| site_id | AC9 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE OXL D1157 |
| Chain | Residue |
| A | ARG96 |
| B | ILE120 |
| B | SER122 |
| B | LEU151 |
| D | ILE120 |
| D | SER122 |
| D | LEU151 |
| F | ARG96 |
| site_id | BC1 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 D1158 |
| Chain | Residue |
| D | ALA39 |
| D | GLU40 |
| D | HOH2299 |
| D | HOH2300 |
| D | HOH2301 |
| D | HOH2302 |
| D | HOH2303 |
| site_id | BC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 D1159 |
| Chain | Residue |
| D | ALA33 |
| D | ASP34 |
| D | GLY35 |
| D | THR36 |
| D | GLY37 |
| D | HOH2110 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 E1157 |
| Chain | Residue |
| E | ALA39 |
| E | GLU40 |
| E | HOH2290 |
| E | HOH2291 |
| E | HOH2292 |
| E | HOH2293 |
| site_id | BC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 E1158 |
| Chain | Residue |
| E | ARG87 |
| E | HOH2294 |
| E | HOH2295 |
| F | ARG87 |
| G | ARG87 |
| G | HOH2212 |
| H | ARG87 |
| site_id | BC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 E1159 |
| Chain | Residue |
| E | ALA33 |
| E | ASP34 |
| E | GLY35 |
| E | THR36 |
| E | GLY37 |
| site_id | BC6 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE OXL F1157 |
| Chain | Residue |
| F | LEU151 |
| G | ARG96 |
| B | ARG96 |
| E | ILE120 |
| E | SER122 |
| E | LEU151 |
| F | ILE120 |
| F | SER122 |
| site_id | BC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 F1158 |
| Chain | Residue |
| F | ALA39 |
| F | GLU40 |
| F | HOH2321 |
| F | HOH2322 |
| F | HOH2323 |
| site_id | BC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 F1159 |
| Chain | Residue |
| F | ALA33 |
| F | ASP34 |
| F | GLY35 |
| F | THR36 |
| F | GLY37 |
| F | HOH2325 |
| F | HOH2326 |
| site_id | BC9 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE OXL G1157 |
| Chain | Residue |
| C | ARG96 |
| E | ARG96 |
| G | ILE120 |
| G | SER122 |
| G | LEU151 |
| H | ILE120 |
| H | SER122 |
| H | LEU151 |
| site_id | CC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 G1158 |
| Chain | Residue |
| G | ALA39 |
| G | GLU40 |
| G | HOH2288 |
| G | HOH2290 |
| site_id | CC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 H1157 |
| Chain | Residue |
| H | ALA39 |
| H | GLU40 |
| H | HOH2277 |
| H | HOH2324 |
| H | HOH2325 |
| H | HOH2326 |
| site_id | CC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 H1158 |
| Chain | Residue |
| H | ASP34 |
| H | GLY35 |
| H | THR36 |
| H | GLY37 |
| H | HOH2142 |
| site_id | CC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE SO4 I1157 |
| Chain | Residue |
| I | ALA39 |
| I | GLU40 |
| I | HOH2324 |
| I | HOH2325 |
| I | HOH2326 |
| I | HOH2328 |
| I | HOH2330 |
| site_id | CC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 I1158 |
| Chain | Residue |
| C | ARG87 |
| C | HOH2219 |
| G | ARG87 |
| H | ARG87 |
| I | ARG87 |
| I | HOH2331 |
| site_id | CC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 I1159 |
| Chain | Residue |
| I | ALA33 |
| I | ASP34 |
| I | GLY35 |
| I | THR36 |
| I | GLY37 |
| site_id | CC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 J1157 |
| Chain | Residue |
| J | ALA39 |
| J | GLU40 |
| J | HOH2370 |
| J | HOH2371 |
| J | HOH2372 |
| site_id | CC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SO4 J1158 |
| Chain | Residue |
| J | ALA33 |
| J | ASP34 |
| J | GLY35 |
| J | THR36 |
| J | GLY37 |
| J | HOH2149 |
| J | HOH2373 |
| J | HOH2374 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 30 |
| Details | Active site: {} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| A | ARG96 | electrostatic stabiliser |
| A | GLU99 | enhance reactivity |
| A | SER122 | electrostatic stabiliser |
| site_id | MCSA10 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| J | ARG96 | electrostatic stabiliser |
| J | GLU99 | enhance reactivity |
| J | SER122 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| B | ARG96 | electrostatic stabiliser |
| B | GLU99 | enhance reactivity |
| B | SER122 | electrostatic stabiliser |
| site_id | MCSA3 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| C | ARG96 | electrostatic stabiliser |
| C | GLU99 | enhance reactivity |
| C | SER122 | electrostatic stabiliser |
| site_id | MCSA4 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| D | ARG96 | electrostatic stabiliser |
| D | GLU99 | enhance reactivity |
| D | SER122 | electrostatic stabiliser |
| site_id | MCSA5 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| E | ARG96 | electrostatic stabiliser |
| E | GLU99 | enhance reactivity |
| E | SER122 | electrostatic stabiliser |
| site_id | MCSA6 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| F | ARG96 | electrostatic stabiliser |
| F | GLU99 | enhance reactivity |
| F | SER122 | electrostatic stabiliser |
| site_id | MCSA7 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| G | ARG96 | electrostatic stabiliser |
| G | GLU99 | enhance reactivity |
| G | SER122 | electrostatic stabiliser |
| site_id | MCSA8 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| H | ARG96 | electrostatic stabiliser |
| H | GLU99 | enhance reactivity |
| H | SER122 | electrostatic stabiliser |
| site_id | MCSA9 |
| Number of Residues | 3 |
| Details | M-CSA 845 |
| Chain | Residue | Details |
| I | ARG96 | electrostatic stabiliser |
| I | GLU99 | enhance reactivity |
| I | SER122 | electrostatic stabiliser |
