2IPL

Crystal structure of a disulfide mutant glucose binding protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	D-galactose-binding periplasmic protein	polymer	318	34563.9	1	UniProt (P0AEE5) Pfam (PF13407) In PDB	Escherichia coli	GBP, D-galactose/D-glucose-binding protein, GGBP
2	A	beta-D-glucopyranose	non-polymer		180.2	1	Chemie (BGC)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4		water	water		18.0	439	Chemie (HOH)

Sequence modifications

A: 1 - 309 (UniProt: P0AEE5)

PDB	External Database	Details
Met 0	-	initiating methionine
Cys 70	Pro 93	engineered mutation
Cys 151	Gly 174	engineered mutation
Gly 310	-	expression tag
Ser 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	34563.9
Non-Polymers*	Number of molecules	2
	Total formula weight	220.2
All*	Total formula weight	34784.2

*Water molecules are not included.