2IPL
Crystal structure of a disulfide mutant glucose binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-11 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.840, 36.680, 79.070 |
Unit cell angles | 90.00, 124.03, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.200 |
R-factor | 0.168 |
Rwork | 0.167 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hph |
RMSD bond length | 0.007 |
RMSD bond angle | 1.196 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.300 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.054 | 0.262 |
Number of reflections | 81424 | |
<I/σ(I)> | 13.17 | 3.7 |
Completeness [%] | 91.6 | 72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M HEPES, pH7.5, 20% PEG 1500, VAPOR DIFFUSION, HANGING DROP, temperature 290K |