2I82

Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (E, F, G, H)	5'-R(*GP*AP*GP*GP*GP*GP*AP*UP*UP*GP*AP*AP*AP*AP*UP*CP*CP*CP*CP*UP*C)-3'	polymer	21	6642.0	4
2	E, F, G, H (A, B, C, D)	Ribosomal large subunit pseudouridine synthase A	polymer	217	25083.6	4	UniProt (P0AA37) Pfam (PF00849)	Escherichia coli	rRNA-uridine lyase A, rRNA pseudouridylate synthase A
3	I, J (E, G)	(5S,6R)-5-FLUORO-6-HYDROXYDIHYDROPYRIMIDINE-2,4(1H,3H)-DIONE	non-polymer		150.1	2	Chemie (FOU)
4	K, L, M, N, O... (E, F, G, H, A...)	water	water		18.0	255	Chemie (HOH)

Sequence modifications

A, B, C, D: 3 - 219 (UniProt: P0AA37)

PDB	External Database	Details
Mse 3	Met 2	modified residue
Mse 24	Met 23	modified residue
Mse 47	Met 46	modified residue
Mse 65	Met 64	modified residue
Mse 168	Met 167	modified residue
Mse 189	Met 188	modified residue
Mse 199	Met 198	modified residue
Mse 211	Met 210	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	126902.4
Non-Polymers*	Number of molecules	2
	Total formula weight	300.2
All*	Total formula weight	127202.6

*Water molecules are not included.