2I82
Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9001, 0.92035, 0.92014, 0.942411, 0.979877, 0.979647 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 320.737, 51.691, 81.233 |
Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
Resolution | 19.830 - 2.050 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.26300 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.830 | 2.180 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.069 | 0.467 |
Number of reflections | 82366 | |
<I/σ(I)> | 35.8 | 4.84 |
Completeness [%] | 97.8 | 97.2 |
Redundancy | 7.43 | 6.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 25% PEG4000, 100mM Tris-HCl pH 8.5, 25mM MgCl2, 100mM LiCl, 1mM spermine, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG4000 | ||
10 | 1 | 2 | LiCl | ||
11 | 1 | 2 | spermine | ||
2 | 1 | 1 | Tris-HCl | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | LiCl | ||
5 | 1 | 1 | spermine | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG4000 | ||
8 | 1 | 2 | Tris-HCl | ||
9 | 1 | 2 | MgCl2 |