2HVY

Crystal structure of an H/ACA box RNP from Pyrococcus furiosus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (E)	H/ACA RNA	polymer	65	21021.5	1
2	B (A)	Probable tRNA pseudouridine synthase B	polymer	346	39453.9	1	UniProt (Q7LWY0) Pfam (PF08068) Pfam (PF01509) Pfam (PF16198) Pfam (PF01472)	Pyrococcus furiosus	tRNA pseudouridine 55 synthase, Psi55 synthase, tRNA-uridine isomerase, tRNA pseudouridylate synthase
3	C (B)	Small nucleolar rnp similar to gar1	polymer	104	12340.8	1	UniProt (Q8U029) Pfam (PF04410)	Pyrococcus furiosus	Gar1
4	D (C)	Ribosome biogenesis protein Nop10	polymer	60	7214.6	1	UniProt (Q8U1R4) Pfam (PF04135)	Pyrococcus furiosus	Nop10
5	E (D)	50S ribosomal protein L7Ae	polymer	130	14314.6	1	UniProt (Q8U160) Pfam (PF01248)	Pyrococcus furiosus	L7ae
6	F (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
7	G (C)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
8	H, I, J, K, L (E, A, B, C, D)	water	water		18.0	118	Chemie (HOH)

Sequence modifications

A: 4 - 343 (UniProt: Q7LWY0)

PDB	External Database	Details
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

C: 1 - 60 (UniProt: Q8U1R4)

PDB	External Database	Details
Lys 2	Arg 2	engineered mutation

D: 2 - 124 (UniProt: Q8U160)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	Met 1	engineered mutation
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	94345.5
Non-Polymers*	Number of molecules	2
	Total formula weight	572.6
All*	Total formula weight	94918.0

*Water molecules are not included.