2HVY
Crystal structure of an H/ACA box RNP from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.031, 90.950, 114.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.23984 |
| Rwork | 0.238 |
| R-free | 0.27807 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ey4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.045 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.055 | 0.331 |
| Number of reflections | 39234 | |
| <I/σ(I)> | 38 | 6.7 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.8 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 303 | 30% MPD, 35mM CH3COOMg, 10mM ATP, 50mM cacodylate , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | CH3COOMg | ||
| 3 | 1 | 1 | ATP | ||
| 4 | 1 | 1 | cacodylate | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | CH3COOMg | ||
| 8 | 1 | 2 | ATP | ||
| 9 | 1 | 2 | cacodylate |
