2HVY
Crystal structure of an H/ACA box RNP from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.031, 90.950, 114.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.23984 |
Rwork | 0.238 |
R-free | 0.27807 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ey4 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.045 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.055 | 0.331 |
Number of reflections | 39234 | |
<I/σ(I)> | 38 | 6.7 |
Completeness [%] | 99.7 | 100 |
Redundancy | 6.8 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 303 | 30% MPD, 35mM CH3COOMg, 10mM ATP, 50mM cacodylate , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | H2O | ||
2 | 1 | 1 | CH3COOMg | ||
3 | 1 | 1 | ATP | ||
4 | 1 | 1 | cacodylate | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | CH3COOMg | ||
8 | 1 | 2 | ATP | ||
9 | 1 | 2 | cacodylate |