2HOT
Phage selected homeodomain bound to modified DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | 5'-D(*TP*TP*TP*TP*GP*CP*CP*AP*TP*GP*TP*AP*AP*TP*CP*CP*CP*CP*GP*GP*A)-3' | polymer | 21 | 6389.1 | 1 | |||
| 2 | B (D) | 5'-D(*AP*TP*CP*CP*GP*GP*GP*GP*AP*TP*TP*AP*CP*AP*TP*GP*GP*CP*AP*AP*A)-3' | polymer | 21 | 6496.2 | 1 | |||
| 3 | C, D (A, B) | Segmentation polarity homeobox protein engrailed | polymer | 63 | 7516.5 | 2 | UniProt (P02836) Pfam (PF00046) | Drosophila melanogaster (fruit fly) | Homeobox |
| 4 | E (C) | 3-PROP-2-YN-1-YL-1,3-OXAZOLIDIN-2-ONE | non-polymer | 125.1 | 1 | Chemie (P2O) | |||
| 5 | F, G (B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 6 | H, I, J, K (C, D, A, B) | water | water | 18.0 | 80 | Chemie (HOH) |
Sequence modifications
A, B: 0 - 60 (UniProt: P02836)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | cloning artifact |
| Ser -1 | - | cloning artifact |
| Val 45 | Ile 498 | engineered mutation |
| Gly 47 | Ile 500 | engineered mutation |
| Lys 50 | Gln 503 | engineered mutation |
| Met 52 | Lys 505 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 27918.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 309.3 | |
| All* | Total formula weight | 28227.7 |
