2HOT
Phage selected homeodomain bound to modified DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.11588 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.856, 45.046, 73.568 |
| Unit cell angles | 90.00, 118.65, 90.00 |
Refinement procedure
| Resolution | 34.880 - 2.190 |
| R-factor | 0.22424 |
| Rwork | 0.222 |
| R-free | 0.25448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.660 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.270 |
| High resolution limit [Å] | 2.190 | 4.720 | 2.190 |
| Rmerge | 0.040 | 0.022 | 0.293 |
| Number of reflections | 18302 | ||
| <I/σ(I)> | 13.8 | ||
| Completeness [%] | 95.2 | 98.4 | 76.3 |
| Redundancy | 3 | 3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG-400/NH4OAc, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG400 | ||
| 2 | 1 | 1 | NH4OAc | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | NH4OAc | ||
| 5 | 1 | 2 | bis-Tris-Propane | ||
| 6 | 1 | 2 | PEG400 |
