2HFK
Pikromycin thioesterase in complex with product 10-deoxymethynolide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Type I polyketide synthase PikAIV | polymer | 319 | 33771.8 | 2 | UniProt (Q9ZGI2) Pfam (PF00975) | Streptomyces venezuelae | Pikromycin |
| 2 | C, D, H (A, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 3 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | F, I, J, K (A, B) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 4 | Chemie (DMS) | |||
| 5 | G, L (A, B) | (3R,4S,5S,7R,9E,11R,12R)-12-ETHYL-4-HYDROXY-3,5,7,11-TETRAMETHYLOXACYCLODODEC-9-ENE-2,8-DIONE | non-polymer | 296.4 | 2 | Chemie (E4H) | |||
| 6 | M, N (A, B) | water | water | 18.0 | 648 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 298 (UniProt: Q9ZGI2)
| PDB | External Database | Details |
|---|---|---|
| Met -20 | - | cloning artifact |
| Gly -19 | - | cloning artifact |
| Ser -18 | - | cloning artifact |
| Ser -17 | - | cloning artifact |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Ser -10 | - | cloning artifact |
| Ser -9 | - | cloning artifact |
| Gly -8 | - | cloning artifact |
| Leu -7 | - | cloning artifact |
| Val -6 | - | cloning artifact |
| Pro -5 | - | cloning artifact |
| Arg -4 | - | cloning artifact |
| Gly -3 | - | cloning artifact |
| Ser -2 | - | cloning artifact |
| His -1 | - | cloning artifact |
| Met 0 | - | cloning artifact |
| Ala 148 | Ser 1196 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 67543.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1217.8 | |
| All* | Total formula weight | 68761.4 |
