2GIG
Alteration of sequence specificity of the type II restriction endonuclease HINCII through an indirect readout mechanism
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | E, F | 5'-D(*GP*CP*CP*GP*GP*TP*CP*GP*AP*CP*CP*GP*GP*C)-3' | polymer | 14 | 4282.8 | 2 | |||
| 2 | A, B | Type II restriction enzyme HincII | polymer | 257 | 29839.1 | 2 | UniProt (P44413) Pfam (PF09226) UniProt (by SIFTS) (P17743) In PDB | Haemophilus influenzae | Endonuclease HincII, R.HincII |
| 3 | A | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 4 | water | water | 18.0 | 463 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 258 (UniProt: P44413)
| PDB | External Database | Details |
|---|---|---|
| Thr 130 | Arg 130 | conflict |
| Phe 138 | Gln 138 | engineered mutation |
| Trp 173 | Ser 173 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 68243.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 46.0 | |
| All* | Total formula weight | 68289.7 |
