2FMO
Ala177Val mutant of E. coli Methylenetetrahydrofolate Reductase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | 5,10-methylenetetrahydrofolate reductase | polymer | 304 | 34243.9 | 3 | UniProt (P0AEZ1) Pfam (PF02219) | Escherichia coli | |
| 2 | D, E, H, I, L (A, B, C) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 3 | F, J, M (A, B, C) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 3 | Chemie (FAD) | |||
| 4 | G, K, N (A, B, C) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 3 | Chemie (MPD) | |||
| 5 | O, P, Q (A, B, C) | water | water | 18.0 | 259 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 296 (UniProt: P0AEZ1)
| PDB | External Database | Details |
|---|---|---|
| Val 177 | Ala 177 | engineered mutation |
| Leu 297 | - | cloning artifact |
| Glu 298 | - | cloning artifact |
| His 299 | - | expression tag |
| His 300 | - | expression tag |
| His 301 | - | expression tag |
| His 302 | - | expression tag |
| His 303 | - | expression tag |
| His 304 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 102731.7 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 3191.5 | |
| All* | Total formula weight | 105923.2 |
