2FMO

Ala177Val mutant of E. coli Methylenetetrahydrofolate Reductase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	5,10-methylenetetrahydrofolate reductase	polymer	304	34243.9	3	UniProt (P0AEZ1) Pfam (PF02219) In PDB	Escherichia coli
2	A, B, C	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
3	A, B, C	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	3	Chemie (FAD)
4	A, B, C	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	3	Chemie (MPD)
5		water	water		18.0	259	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 296 (UniProt: P0AEZ1)

PDB	External Database	Details
Val 177	Ala 177	engineered mutation
Leu 297	-	cloning artifact
Glu 298	-	cloning artifact
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	102731.7
Non-Polymers*	Number of molecules	11
	Total formula weight	3191.5
All*	Total formula weight	105923.2

*Water molecules are not included.