2FMO
Ala177Val mutant of E. coli Methylenetetrahydrofolate Reductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-10-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.153, 128.350, 97.581 |
Unit cell angles | 90.00, 121.07, 90.00 |
Refinement procedure
Resolution | 20.010 - 2.250 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b5t |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.010 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 50832 | |
<I/σ(I)> | 15.9 | |
Completeness [%] | 99.7 | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 4000, LITHIUM SULFATE, SODIUM CACODYLATE, ETHANOL, METHYLPENTANEDIOL, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |