2F8X

Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (X)	5'-D(*GP*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*AP*AP*A)-3'	polymer	18	5628.7	1
2	B (Y)	5'-D(*TP*TP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*TP*AP*AP*C)-3'	polymer	18	5401.5	1
3	C (C)	Recombining binding protein suppressor of hairless, isoform 4	polymer	434	49294.2	1	UniProt (Q06330) Pfam (PF09271) Pfam (PF09270) Pfam (PF20144)	Homo sapiens (human)
4	D (K)	Neurogenic locus notch homolog protein 1	polymer	256	27885.0	1	UniProt (P46531) Pfam (PF00023) Pfam (PF12796)	Homo sapiens (human)	(Notch 1) (hN1) (Translocation-associated notch protein TAN-1)
5	E (M)	Mastermind-like protein 1	polymer	63	7532.8	1	UniProt (Q92585) Pfam (PF09596)	Homo sapiens (human)	Mam-1
6	F, G, H (C, K, M)	water	water		18.0	10	Chemie (HOH)

Sequence modifications

C: 9 - 435 (UniProt: Q06330)

PDB	External Database	Details
Met 8	-	initiating methionine
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag

K: 1873 - 2127 (UniProt: P46531)

PDB	External Database	Details
Gly 1872	-	cloning artifact

M: 13 - 74 (UniProt: Q92585)

PDB	External Database	Details
Gly 12	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	95742.2
All*	Total formula weight	95742.2

*Water molecules are not included.