2F8X
Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2005-07-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 273.866, 273.866, 121.016 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.820 - 3.250 |
| R-factor | 0.241 |
| Rwork | 0.222 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries: 1TTU and 2F8Y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.820 | 3.450 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Number of reflections | 72848 | |
| <I/σ(I)> | 19.7 | 3.3 |
| Completeness [%] | 91.1 | 89.3 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 7.9 | 298 | 50mM HEPES, 6% PEG 3350, and 5% ethylene glycol, pH 7.9, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | ethylene glycol | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | HEPES | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | ethylene glycol |
