2F5Z

Crystal Structure of Human Dihydrolipoamide Dehydrogenase (E3) Complexed to the E3-Binding Domain of Human E3-Binding Protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Dihydrolipoyl dehydrogenase	polymer	474	50208.4	10	UniProt (P09622) Pfam (PF07992) Pfam (PF02852)	Homo sapiens (human)	Dihydrolipoamide dehydrogenase, Glycine cleavage system L protein
2	K, L, M, N, O (K, L, M, N, O)	Pyruvate dehydrogenase protein X component	polymer	64	7150.2	5	UniProt (O00330) Pfam (PF02817)	Homo sapiens (human)	Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, Lipoyl-containing pyruvate dehydrogenase complex component X, E3-binding protein, E3BP, proX
3	AA, AB, BA, BB, CA... (C, H, I, D, J...)	SULFATE ION	non-polymer		96.1	39	Chemie (SO4)
4	CB, DA, GB, IA, LB... (H, C, I, D, J...)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	10	Chemie (FAD)
5	MB, NB, OB, PB, QB... (A, B, C, D, E...)	water	water		18.0	1776	Chemie (HOH)

Sequence modifications

K, L, M, N, O: 120 - 177 (UniProt: O00330)

PDB	External Database	Details
Gly 120	Lys 173	engineered mutation
Leu 176	Thr 229	engineered mutation
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	537834.8
Non-Polymers*	Number of molecules	49
	Total formula weight	11602.0
All*	Total formula weight	549436.8

*Water molecules are not included.