2F5Z

Crystal Structure of Human Dihydrolipoamide Dehydrogenase (E3) Complexed to the E3-Binding Domain of Human E3-Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-09
Detector	CUSTOM-MADE
Wavelength(s)	0.979426
Spacegroup name	P 21 21 21
Unit cell lengths	171.153, 187.728, 224.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.400 - 2.180
R-factor	0.205
Rwork	0.205
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ZMD without NADH
RMSD bond length	0.011
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.400	2.260
High resolution limit [Å]	2.180	2.180
Number of reflections	367237
<I/σ(I)>	21.9	1.9
Completeness [%]	98.2	84.8
Redundancy	3.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	14% (w/v) PEG4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, 0.03 M spermidine, 8% (v/v) glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K