2ERJ

Crystal structure of the heterotrimeric interleukin-2 receptor in complex with interleukin-2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Interleukin-2 receptor alpha chain	polymer	225	25402.4	2	UniProt (P01589) Pfam (PF00084)	Homo sapiens (human)	IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, CD25, TAC antigen
2	B, F (B, F)	Interleukin-2 receptor beta chain	polymer	219	25452.9	2	UniProt (P14784) Pfam (PF18707)	Homo sapiens (human)	IL-2 receptor beta subunit, IL-2-RB, p75, CD122
3	C, G (C, G)	Cytokine receptor common gamma chain	polymer	247	29169.5	2	UniProt (P31785) Pfam (PF22012) Pfam (PF21605)	Homo sapiens (human)	IL-2 receptor Gamma subunit, IL-2-RG, p65, CD132
4	D, H (D, H)	Interleukin-2	polymer	133	15403.9	2	UniProt (P60568) Pfam (PF00715)	Homo sapiens (human)	IL-2, T-cell growth factor, TCGF, Aldesleukin
5	I, M (I, M)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	2	In PDB GlyTouCan (G32152BH)
6	J (J)	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G86851RC)
7	K, O (K, O)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
8	L, P (L, P)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
9	N (N)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
10	Q, R, S, T (C, G)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

A, E: 1 - 212 (UniProt: P01589)

PDB	External Database	Details
Gly -4	-	cloning artifact
Met -3	-	cloning artifact
Leu -2	-	cloning artifact
Ser -1	-	cloning artifact
Leu 0	-	cloning artifact
Ser 49	Asn 70	engineered mutation
Ser 68	Asn 89	engineered mutation
Thr 213	-	expression tag
Gly 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag

B, F: 1 - 206 (UniProt: P14784)

PDB	External Database	Details
Gly -4	-	cloning artifact
Met -3	-	cloning artifact
Leu -2	-	cloning artifact
Ser -1	-	cloning artifact
Leu 0	-	cloning artifact
Thr 207	-	expression tag
Gly 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag

C, G: 1 - 233 (UniProt: P31785)

PDB	External Database	Details
Gly -4	-	cloning artifact
Met -3	-	cloning artifact
Leu -2	-	cloning artifact
Ser -1	-	cloning artifact
Leu 0	-	cloning artifact
Gln 53	Asn 75	engineered mutation
Arg 234	-	expression tag
Thr 235	-	expression tag
Gly 236	-	expression tag
His 237	-	expression tag
His 238	-	expression tag
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag

D, H: 1 - 133 (UniProt: P60568)

PDB	External Database	Details
Ala 125	Cys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	190857.5
Branched	Number of molecules	8
	Total formula weight	4425.1
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	196167.4

*Water molecules are not included.