2ERJ
Crystal structure of the heterotrimeric interleukin-2 receptor in complex with interleukin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.266, 70.545, 129.236 |
| Unit cell angles | 83.85, 82.45, 89.72 |
Refinement procedure
| Resolution | 129.100 - 3.000 |
| R-factor | 0.22254 |
| Rwork | 0.220 |
| R-free | 0.26288 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB structure 2B5I |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 129.100 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 37249 | |
| <I/σ(I)> | 7.8 | 1.7 |
| Completeness [%] | 94.4 | 75.3 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 295.5 | MPEG 550, sodium acetate, sodium citrate, phenol, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |
