2D0K
Methionine-free mutant of Escherichia coli dihydrofolate reductase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Dihydrofolate reductase | polymer | 159 | 17923.9 | 2 | UniProt (P00379) Pfam (PF00186) UniProt (by SIFTS) (P0ABQ4) | Escherichia coli | |
| 2 | C, E (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 3 | D, F (A, B) | FOLIC ACID | non-polymer | 441.4 | 2 | Chemie (FOL) | |||
| 4 | G, H (A, B) | water | water | 18.0 | 206 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 159 (UniProt: P00379)
| PDB | External Database | Details |
|---|---|---|
| Ala 1 | Met 1 | engineered mutation |
| Asn 16 | Met 16 | engineered mutation |
| Leu 20 | Met 20 | engineered mutation |
| Tyr 42 | Met 42 | engineered mutation |
| Ala 85 | Cys 85 | engineered mutation |
| Phe 92 | Met 92 | engineered mutation |
| Ser 152 | Cys 152 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 35847.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 953.7 | |
| All* | Total formula weight | 36801.5 |
