2D0K
Methionine-free mutant of Escherichia coli dihydrofolate reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 290 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2000-02-04 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.580, 56.690, 85.140 |
| Unit cell angles | 90.00, 106.81, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dyi |
| RMSD bond length | 0.070 |
| RMSD bond angle | 1.347 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.030 | 0.228 |
| Number of reflections | 25648 | |
| Completeness [%] | 90.4 | 78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | PEG6000, Magnesium chroride, Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
