2D0G
Crystal Structure of Thermoactinomyces vulgaris R-47 Alpha-Amylase 1 (TVAI) Mutant D356N/E396Q complexed with P5, a pullulan model oligosaccharide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | alpha-amylase I | polymer | 637 | 71108.3 | 1 | UniProt (Q60053) Pfam (PF02903) Pfam (PF00128) In PDB | Thermoactinomyces vulgaris | TVA I |
| 2 | B | beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 1639.4 | 1 | In PDB GlyTouCan (G39844GI) | |||
| 3 | C | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900005) GlyTouCan (G84824ZO) | beta-cellobiose | ||
| 4 | D | alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-6)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 828.7 | 1 | In PDB GlyTouCan (G95107LS) | |||
| 5 | A | alpha-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (GLC) | |||
| 6 | A | beta-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (BGC) | |||
| 7 | A | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 8 | A | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 1 | Chemie (MPD) | |||
| 9 | water | water | 18.0 | 474 | Chemie (HOH) |
Sequence modifications
A: 1 - 637 (UniProt: Q60053)
| PDB | External Database | Details |
|---|---|---|
| Asn 356 | Asp 385 | engineered mutation |
| Gln 396 | Glu 425 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 71108.3 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 2810.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 598.7 | |
| All* | Total formula weight | 74517.5 |
