2D0G
Crystal Structure of Thermoactinomyces vulgaris R-47 Alpha-Amylase 1 (TVAI) Mutant D356N/E396Q complexed with P5, a pullulan model oligosaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.885, 50.698, 108.007 |
Unit cell angles | 90.00, 103.43, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.600 |
R-factor | 0.154 |
Rwork | 0.150 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.355 | 2.730 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 19275 | |
Completeness [%] | 96.8 | 83.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 3% PEG20000, 0.05M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |