2C7R

HhaI DNA methyltransferase (T250G mutant) complex with oligonucleotide containing 2-aminopurine as a target base (GPGC:GMGC) and SAH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	MODIFICATION METHYLASE HHAI	polymer	327	36998.2	1	UniProt (P05102)	HAEMOPHILUS HAEMOLYTICUS	DNA METHYLTRANSFERASE HHAI, CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI
2	B (C)	5'-D(*G*GP*AP*TP*GP*(5CM)*GP*CP*TP*GP*AP*C)-3'	polymer	12	3717.4	1		SYNTHETIC CONSTRUCT
3	C (D)	5'-D(*G*TP*CP*AP*GP*(2PR)*GP*CP*AP*TP*CP*C)-3'	polymer	12	3647.4	1		SYNTHETIC CONSTRUCT
4	D (A)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH) PubChem (16760024) PubChem (25210495) PubChem (177678424) PubChem (21125603) PubChem (25203782) PubChem (52224400) PubChem (44361184) PubChem (46936206) PubChem (50989525) PubChem (51380914) PubChem (6419189) PubChem (52224398) PubChem (57544532) PubChem (164157219) PubChem (59046843) PubChem (59170628) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (118374891) PubChem (118560068) PubChem (193) PubChem (13792) PubChem (189149) PubChem (439155) PubChem (5284331) PubChem (126969526) PubChem (71170554) PubChem (129105892) PubChem (13231890) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525)
5	E, F, G, H, I... (A)	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
6	L, M, N, O, P (A)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL) PubChem (753)
7	Q, R, S (A, C, D)	water	water		18.0	271	Chemie (HOH)

Sequence modifications

A: 1 - 327 (UniProt: P05102)

PDB	External Database	Details
Gly 250	Thr 250	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	44363.0
Non-Polymers*	Number of molecules	13
	Total formula weight	1517.3
All*	Total formula weight	45880.3

*Water molecules are not included.