2C7R
HhaI DNA methyltransferase (T250G mutant) complex with oligonucleotide containing 2-aminopurine as a target base (GPGC:GMGC) and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID13 |
Synchrotron site | ESRF |
Beamline | ID13 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | H 3 2 |
Unit cell lengths | 95.620, 95.620, 315.756 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.500 - 1.900 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mht |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.240 |
Number of reflections | 44234 | |
<I/σ(I)> | 8.4 | 2.8 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 50 MM NA CITRATE PH 5.6, 1.7 M AMMONIUM SULFATE, 5 % GLUCOSE |