2C7R
HhaI DNA methyltransferase (T250G mutant) complex with oligonucleotide containing 2-aminopurine as a target base (GPGC:GMGC) and SAH
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003886 | molecular_function | DNA (cytosine-5-)-methyltransferase activity |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009307 | biological_process | DNA restriction-modification system |
| A | 0016740 | molecular_function | transferase activity |
| A | 0032259 | biological_process | methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1329 |
| Chain | Residue |
| A | ASP199 |
| A | HOH2118 |
| A | HOH2172 |
| A | HOH2218 |
| A | HOH2219 |
| A | HOH2220 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1330 |
| Chain | Residue |
| A | PRO286 |
| A | ASP287 |
| A | SER288 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1331 |
| Chain | Residue |
| A | LYS193 |
| A | ARG245 |
| A | LYS290 |
| A | HOH2151 |
| A | HOH2173 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1332 |
| Chain | Residue |
| A | LEU196 |
| A | LEU197 |
| A | PRO198 |
| A | ARG281 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1333 |
| Chain | Residue |
| A | LYS114 |
| A | ARG172 |
| A | HOH2222 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1334 |
| Chain | Residue |
| A | GLU220 |
| A | GLN221 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE SO4 A 1335 |
| Chain | Residue |
| A | MET51 |
| A | GLY269 |
| A | PRO293 |
| site_id | AC8 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE SAH A 1328 |
| Chain | Residue |
| A | PHE18 |
| A | ALA19 |
| A | LEU21 |
| A | GLY23 |
| A | ASN39 |
| A | GLU40 |
| A | TRP41 |
| A | ASP42 |
| A | ASP60 |
| A | ILE61 |
| A | GLY78 |
| A | PRO80 |
| A | LEU100 |
| A | TYR285 |
| A | ASN304 |
| A | SER305 |
| A | VAL306 |
| A | HOH2043 |
| A | HOH2189 |
| A | HOH2191 |
| A | HOH2213 |
| A | HOH2215 |
| A | HOH2216 |
| A | HOH2217 |
| site_id | AC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL A 1336 |
| Chain | Residue |
| A | LYS150 |
| A | VAL151 |
| A | LEU152 |
| A | ASN153 |
| A | ASP156 |
| A | TYR157 |
| A | PRO185 |
| A | HOH2084 |
| A | HOH2224 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL A 1337 |
| Chain | Residue |
| A | GLU3 |
| A | ILE4 |
| A | LYS5 |
| A | ASP6 |
| A | TYR316 |
| A | TYR327 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE GOL A 1338 |
| Chain | Residue |
| A | TYR316 |
| A | LYS325 |
| A | PRO326 |
| A | TYR327 |
| site_id | BC3 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GOL A 1339 |
| Chain | Residue |
| A | GLN237 |
| A | GLY238 |
| A | GLY250 |
| A | LYS261 |
| A | THR262 |
| A | GLY263 |
| A | HOH2148 |
| A | HOH2149 |
| A | HOH2153 |
| A | HOH2225 |
| site_id | BC4 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL A 1340 |
| Chain | Residue |
| A | GLU203 |
| A | HIS275 |
| A | HOH2218 |
| A | HOH2226 |
| A | HOH2227 |
| A | HOH2228 |
| A | HOH2229 |
Functional Information from PROSITE/UniProt
| site_id | PS00094 |
| Number of Residues | 13 |
| Details | C5_MTASE_1 C-5 cytosine-specific DNA methylases active site. DiLcaGfPCqAFS |
| Chain | Residue | Details |
| A | ASP73-SER85 |
| site_id | PS00095 |
| Number of Residues | 19 |
| Details | C5_MTASE_2 C-5 cytosine-specific DNA methylases C-terminal signature. KqfGNSVvInVlqyIaynI |
| Chain | Residue | Details |
| A | LYS300-ILE318 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 313 |
| Details | Domain: {"description":"SAM-dependent MTase C5-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU01016","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Active site: {"evidences":[{"source":"PubMed","id":"7899082","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mht |
| Chain | Residue | Details |
| A | CYS81 | |
| A | GLU119 |
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 293 |
| Chain | Residue | Details |
| A | CYS81 | covalently attached, hydrogen bond acceptor, nucleofuge, nucleophile, polar interaction, proton acceptor, proton donor |
| A | GLU119 | attractive charge-charge interaction, electrostatic stabiliser, hydrogen bond acceptor, increase electrophilicity |
| A | ARG163 | attractive charge-charge interaction, electrostatic stabiliser, hydrogen bond donor, increase electrophilicity |
| A | ARG165 | attractive charge-charge interaction, electrostatic stabiliser, hydrogen bond donor, increase electrophilicity |
