2BD1
A possible role of the second calcium ion in interfacial binding: Atomic and medium resolution crystal structures of the quadruple mutant of phospholipase A2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Phospholipase A2 | polymer | 123 | 13818.6 | 2 | UniProt (P00593) Pfam (PF00068) | Bos taurus (cattle) | Phosphatidylcholine 2-acylhydrolase, Group IB phospholipase A2 |
| 2 | C, D, F, G (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 3 | E, H (A, B) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 2 | Chemie (MPD) | |||
| 4 | I, J (A, B) | water | water | 18.0 | 218 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 123 (UniProt: P00593)
| PDB | External Database | Details |
|---|---|---|
| Met 53 | Lys 75 | engineered mutation |
| Met 56 | Lys 78 | engineered mutation |
| Met 120 | Lys 142 | engineered mutation |
| Met 121 | Lys 143 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 27637.1 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 396.7 | |
| All* | Total formula weight | 28033.8 |
