2ARY

Catalytic domain of Human Calpain-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Calpain-1 catalytic subunit	polymer	351	39578.5	2	UniProt (P07384) Pfam (PF00648)	Homo sapiens (human)	Calpain-1 large subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, muCANP, Micromolar-calpain
2	C, D, F, G (A, B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
3	E, H, I (A, B)	BETA-MERCAPTOETHANOL	non-polymer		78.1	3	Chemie (BME)
4	J, K (A, B)	water	water		18.0	229	Chemie (HOH)

Sequence modifications

A, B: 29 - 360 (UniProt: P07384)

PDB	External Database	Details
Met 10	-	cloning artifact
Gly 11	-	cloning artifact
Ser 12	-	cloning artifact
Ser 13	-	cloning artifact
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
Ser 20	-	cloning artifact
Ser 21	-	cloning artifact
Gly 22	-	cloning artifact
Leu 23	-	cloning artifact
Val 24	-	cloning artifact
Pro 25	-	cloning artifact
Arg 26	-	cloning artifact
Gly 27	-	cloning artifact
Ser 28	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	79156.9
Non-Polymers*	Number of molecules	7
	Total formula weight	394.7
All*	Total formula weight	79551.6

*Water molecules are not included.