2A19

PKR kinase domain- eIF2alpha- AMP-PNP complex.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Eukaryotic translation initiation factor 2 alpha subunit	polymer	175	20553.8	1	UniProt (P20459) Pfam (PF00575) Pfam (PF07541) In PDB	Saccharomyces cerevisiae (baker's yeast)	eIF-2- alpha
2	B, C	Interferon-induced, double-stranded RNA-activated protein kinase	polymer	284	32799.6	2	UniProt (P19525) Pfam (PF00069) In PDB	Homo sapiens (human)	Interferon-inducible RNA-dependent protein kinase, p68 kinase, P1/eIF-2A protein kinase
3	B, C	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	B, C	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
5	C	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6		water	water		18.0	72	Chemie (HOH)

Sequence modifications

A: 3 - 175 (UniProt: P20459)

PDB	External Database	Details
Gly 1	-	cloning artifact
Ser 2	-	cloning artifact

B, C: 258 - 538 (UniProt: P19525)

PDB	External Database	Details
Gly 255	-	cloning artifact
Ala 256	-	cloning artifact
His 257	-	cloning artifact
-	Asp 338	deletion
-	Asp 339	deletion
-	Ser 340	deletion
-	Leu 341	deletion
-	Glu 342	deletion
-	Ser 343	deletion
-	Ser 344	deletion
-	Asp 345	deletion
-	Tyr 346	deletion
-	Asp 347	deletion
-	Pro 348	deletion
-	Glu 349	deletion
-	Asn 350	deletion
Asn 412	His 412	engineered mutation
Tpo 446	Thr 446	modified residue
Ala 551	Cys 551	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	86152.9
Non-Polymers*	Number of molecules	7
	Total formula weight	1204.6
All*	Total formula weight	87357.5

*Water molecules are not included.