2A19

PKR kinase domain- eIF2alpha- AMP-PNP complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-29
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
Unit cell lengths	64.659, 48.750, 133.434
Unit cell angles	90.00, 98.44, 90.00

Refinement procedure

Resolution	27.330 - 2.500
R-factor	0.23394
Rwork	0.228
R-free	0.28644
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a1a
RMSD bond length	0.010
RMSD bond angle	1.327
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	28956
<I/σ(I)>	27.8	3.2
Completeness [%]	98.7	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1