2A19
PKR kinase domain- eIF2alpha- AMP-PNP complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-29 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.659, 48.750, 133.434 |
Unit cell angles | 90.00, 98.44, 90.00 |
Refinement procedure
Resolution | 27.330 - 2.500 |
R-factor | 0.23394 |
Rwork | 0.228 |
R-free | 0.28644 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2a1a |
RMSD bond length | 0.010 |
RMSD bond angle | 1.327 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 28956 | |
<I/σ(I)> | 27.8 | 3.2 |
Completeness [%] | 98.7 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |