29VJ

Crystal structure of the human METTL3-METTL14 in complex with STM-2457

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	N6-adenosine-methyltransferase catalytic subunit	polymer	228	26017.8	1	UniProt (Q86U44) Pfam (PF05063)	Homo sapiens (human)	Methyltransferase-like protein 3,hMETTL3,N6-adenosine-methyltransferase 70 kDa subunit,MT-A70
2	B (B)	N(6)-adenosine-methyltransferase non-catalytic subunit METTL14	polymer	307	35690.4	1	UniProt (Q9HCE5) Pfam (PF05063)	Homo sapiens (human)	Methyltransferase-like protein 14,hMETTL14
3	C (A)	~{N}-[[6-[(cyclohexylmethylamino)methyl]imidazo[1,2-a]pyridin-2-yl]methyl]-4-oxidanylidene-1~{H}-pyrido[1,2-a]pyrimidine-2-carboxamide	non-polymer		445.5	1	Chemie (V22)
4	D (B)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT) PubChem (175)
5	E, F (A, B)	water	water		18.0	104	Chemie (HOH)

Sequence modifications

A: 354 - 580 (UniProt: Q86U44)

PDB	External Database	Details
Met 353	-	initiating methionine

B: 107 - 395 (UniProt: Q9HCE5)

PDB	External Database	Details
Met 89	-	initiating methionine
His 90	-	expression tag
His 91	-	expression tag
His 92	-	expression tag
His 93	-	expression tag
His 94	-	expression tag
His 95	-	expression tag
Ser 96	-	expression tag
Ser 97	-	expression tag
Gly 98	-	expression tag
Arg 99	-	expression tag
Glu 100	-	expression tag
Asn 101	-	expression tag
Leu 102	-	expression tag
Tyr 103	-	expression tag
Phe 104	-	expression tag
Gln 105	-	expression tag
Gly 106	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61708.3
Non-Polymers*	Number of molecules	2
	Total formula weight	504.6
All*	Total formula weight	62212.9

*Water molecules are not included.