29VJ

Crystal structure of the human METTL3-METTL14 in complex with STM-2457

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 32 2 1
Unit cell lengths	63.780, 63.780, 224.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.440 - 2.110
R-factor	0.2058
Rwork	0.204
R-free	0.24060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.929
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	1.830
High resolution limit [Å]	1.730	1.730
Number of reflections	57292	8981
<I/σ(I)>	19.12
Completeness [%]	99.7
Redundancy	7.8
CC(1/2)	0.999	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 0.4M Magnesium acetate