28JV
Cryo-EM structure of the human holo-TFIIH-XPC complex bound to bulky lesion-mimic DNA (consensus map)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | General transcription and DNA repair factor IIH helicase subunit XPB | polymer | 797 | 91300.7 | 1 | UniProt (P19447) Pfam (PF13625) Pfam (PF04851) Pfam (PF16203) | Homo sapiens (human) | TFIIH subunit XPB,Basic transcription factor 2 89 kDa subunit,BTF2 p89,DNA excision repair protein ERCC-3,DNA repair protein complementing XP-B cells,TFIIH basal transcription factor complex 89 kDa subunit,TFIIH p89,Xeroderma pigmentosum group B-complementing protein |
| 2 | B (B) | TFIIH basal transcription factor complex helicase XPD subunit | polymer | 771 | 88306.3 | 1 | UniProt (P18074) Pfam (PF06733) Pfam (PF06777) Pfam (PF13307) | Homo sapiens (human) | Basic transcription factor 2 80 kDa subunit,BTF2 p80,CXPD,DNA excision repair protein ERCC-2,DNA repair protein complementing XP-D cells,TFIIH basal transcription factor complex 80 kDa subunit,TFIIH p80,Xeroderma pigmentosum group D-complementing protein |
| 3 | C (C) | General transcription factor IIH subunit 1 | polymer | 591 | 66404.0 | 1 | UniProt (P32780) Pfam (PF08567) Pfam (PF03909) | Homo sapiens (human) | Basic transcription factor 2 62 kDa subunit,BTF2 p62,General transcription factor IIH polypeptide 1,TFIIH basal transcription factor complex p62 subunit |
| 4 | D (D) | General transcription factor IIH subunit 4 | polymer | 462 | 52245.2 | 1 | UniProt (Q92759) Pfam (PF18307) Pfam (PF03849) | Homo sapiens (human) | Basic transcription factor 2 52 kDa subunit,BTF2 p52,General transcription factor IIH polypeptide 4,TFIIH basal transcription factor complex p52 subunit |
| 5 | E (E) | General transcription factor IIH subunit 2 | polymer | 395 | 44482.0 | 1 | UniProt (Q13888) Pfam (PF04056) Pfam (PF07975) | Homo sapiens (human) | Basic transcription factor 2 44 kDa subunit,BTF2 p44,General transcription factor IIH polypeptide 2,TFIIH basal transcription factor complex p44 subunit |
| 6 | F (F) | General transcription factor IIH subunit 3 | polymer | 308 | 34416.0 | 1 | UniProt (Q13889) Pfam (PF03850) | Homo sapiens (human) | Basic transcription factor 2 34 kDa subunit,BTF2 p34,General transcription factor IIH polypeptide 3,TFIIH basal transcription factor complex p34 subunit |
| 7 | G (G) | General transcription factor IIH subunit 5 | polymer | 71 | 8060.4 | 1 | UniProt (Q6ZYL4) Pfam (PF06331) | Homo sapiens (human) | General transcription factor IIH polypeptide 5,TFB5 ortholog,TFIIH basal transcription factor complex TTD-A subunit |
| 8 | H (H) | CDK-activating kinase assembly factor MAT1 | polymer | 309 | 35874.0 | 1 | UniProt (P51948) Pfam (PF17121) Pfam (PF06391) Pfam (PF25811) | Homo sapiens (human) | CDK7/cyclin-H assembly factor,Cyclin-G1-interacting protein,Menage a trois,RING finger protein 66,RING finger protein MAT1,p35,p36 |
| 9 | I (I) | DNA repair protein complementing XP-C cells | polymer | 940 | 106142.2 | 1 | UniProt (Q01831) Pfam (PF03835) Pfam (PF10403) Pfam (PF10404) Pfam (PF10405) | Homo sapiens (human) | Xeroderma pigmentosum group C-complementing protein,p125 |
| 10 | J (J) | Lysine-specific demethylase RAD23B | polymer | 409 | 43203.9 | 1 | UniProt (P54727) Pfam (PF00240) Pfam (PF00627) Pfam (PF09280) | Homo sapiens (human) | UV excision repair protein RAD23 homolog B,HR23B,hHR23B,XP-C repair-complementing complex 58 kDa protein,p58 |
| 11 | K (K) | Centrin-2 | polymer | 172 | 19769.5 | 1 | UniProt (P41208) Pfam (PF13499) | Homo sapiens (human) | Caltractin isoform 1 |
| 12 | L (L) | DNA (Cy5) | polymer | 71 | 21483.1 | 1 | synthetic construct | ||
| 13 | M (M) | DNA (biotinylated) | polymer | 92 | 29197.7 | 1 | synthetic construct | ||
| 14 | N (B) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 15 | O, P, Q, R, S (E, F) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) PubChem (32051) | |||
| 16 | T, U (K) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) PubChem (271) |
Sequence modifications
A: 1 - 782 (UniProt: P19447)
B: 1 - 760 (UniProt: P18074)
C: 1 - 548 (UniProt: P32780)
| PDB | External Database | Details |
|---|---|---|
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 761 | - | expression tag |
| Ser 762 | - | expression tag |
| Asn 763 | - | expression tag |
| Asp 764 | - | expression tag |
| Tyr 765 | - | expression tag |
| Lys 766 | - | expression tag |
| Asp 767 | - | expression tag |
| Asp 768 | - | expression tag |
| Asp 769 | - | expression tag |
| Asp 770 | - | expression tag |
| Lys 771 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 549 | - | expression tag |
| Ser 550 | - | expression tag |
| Gly 551 | - | expression tag |
| Gly 552 | - | expression tag |
| Glu 553 | - | expression tag |
| Asn 554 | - | expression tag |
| Leu 555 | - | expression tag |
| Tyr 556 | - | expression tag |
| Phe 557 | - | expression tag |
| Gln 558 | - | expression tag |
| Ser 559 | - | expression tag |
| Gly 560 | - | expression tag |
| Ser 561 | - | expression tag |
| Trp 562 | - | expression tag |
| Ser 563 | - | expression tag |
| His 564 | - | expression tag |
| Pro 565 | - | expression tag |
| Gln 566 | - | expression tag |
| Phe 567 | - | expression tag |
| Glu 568 | - | expression tag |
| Lys 569 | - | expression tag |
| Gly 570 | - | expression tag |
| Gly 571 | - | expression tag |
| Gly 572 | - | expression tag |
| Ser 573 | - | expression tag |
| Gly 574 | - | expression tag |
| Gly 575 | - | expression tag |
| Gly 576 | - | expression tag |
| Ser 577 | - | expression tag |
| Gly 578 | - | expression tag |
| Gly 579 | - | expression tag |
| Gly 580 | - | expression tag |
| Ser 581 | - | expression tag |
| Trp 582 | - | expression tag |
| Ser 583 | - | expression tag |
| His 584 | - | expression tag |
| Pro 585 | - | expression tag |
| Gln 586 | - | expression tag |
| Phe 587 | - | expression tag |
| Glu 588 | - | expression tag |
| Lys 589 | - | expression tag |
| Ser 590 | - | expression tag |
| Gly 591 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 640884.8 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 758.8 | |
| All* | Total formula weight | 641643.7 |
