1ZNV

How a His-metal finger endonuclease ColE7 binds and cleaves DNA with a transition metal ion cofactor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Colicin E7 immunity protein	polymer	93	10735.8	2	UniProt (Q03708) Pfam (PF01320)	Escherichia coli str. K12 substr.	ImmE7, Microcin E7 immunity protein
2	B, D (B, D)	Colicin E7	polymer	134	15400.5	2	UniProt (Q47112) Pfam (PF21431)	Escherichia coli str. K12 substr.
3	E, G (B, D)	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)
4	F, H (B, D)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	I, J, K, L (A, B, C, D)	water	water		18.0	423	Chemie (HOH)

Sequence modifications

A, C: 1 - 87 (UniProt: Q03708)

PDB	External Database	Details
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
His 91	-	expression tag
His 92	-	expression tag
His 93	-	expression tag

B, D: 444 - 576 (UniProt: Q47112)

PDB	External Database	Details
Met 443	-	initiating methionine
Glu 545	His 545	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52272.6
Non-Polymers*	Number of molecules	4
	Total formula weight	307.3
All*	Total formula weight	52579.9

*Water molecules are not included.