1ZNV
How a His-metal finger endonuclease ColE7 binds and cleaves DNA with a transition metal ion cofactor
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | Colicin E7 immunity protein | polymer | 93 | 10735.8 | 2 | UniProt (Q03708) Pfam (PF01320) In PDB | Escherichia coli str. K12 substr. | ImmE7, Microcin E7 immunity protein |
2 | B, D | Colicin E7 | polymer | 134 | 15400.5 | 2 | UniProt (Q47112) Pfam (PF21431) In PDB | Escherichia coli str. K12 substr. | |
3 | B, D | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) | |||
4 | B, D | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
5 | water | water | 18.0 | 423 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 87 (UniProt: Q03708)
B, D: 444 - 576 (UniProt: Q47112)
PDB | External Database | Details |
---|---|---|
His 88 | - | expression tag |
His 89 | - | expression tag |
His 90 | - | expression tag |
His 91 | - | expression tag |
His 92 | - | expression tag |
His 93 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met 443 | - | initiating methionine |
Glu 545 | His 545 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 52272.6 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 307.3 | |
All* | Total formula weight | 52579.9 |