1ZNV
How a His-metal finger endonuclease ColE7 binds and cleaves DNA with a transition metal ion cofactor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2004-12-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 119.021, 62.882, 74.769 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.650 - 2.000 |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.23500 |
Structure solution method | Isomorphous to 1MZ8 |
Starting model (for MR) | 1mz8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 223845 | |
<I/σ(I)> | 25.34 | 5.73 |
Completeness [%] | 99.8 | |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 0.3M phosphate buffer, 50mM NaCl, 20% PEG550 MME, 10% glycerol, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |