1WSF

Co-crystal structure of E.coli RNase HI active site mutant (D134A*) with Mn2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Ribonuclease HI	polymer	155	17520.9	4	UniProt (P0A7Y4) Pfam (PF00075)	Escherichia coli	RNase HI, Ribonuclease H, RNase H
2	E, F, G (A, B, D)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
3	H, I, J, K (A, B, C, D)	water	water		18.0	174	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 155 (UniProt: P0A7Y4)

PDB	External Database	Details
Ala 87	Lys 87	engineered mutation
Ala 134	Asp 134	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	70083.5
Non-Polymers*	Number of molecules	3
	Total formula weight	164.8
All*	Total formula weight	70248.4

*Water molecules are not included.