1WSF
Co-crystal structure of E.coli RNase HI active site mutant (D134A*) with Mn2+
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004523 | molecular_function | RNA-DNA hybrid ribonuclease activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006401 | biological_process | RNA catabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0043137 | biological_process | DNA replication, removal of RNA primer |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003676 | molecular_function | nucleic acid binding |
| B | 0004518 | molecular_function | nuclease activity |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0004523 | molecular_function | RNA-DNA hybrid ribonuclease activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006401 | biological_process | RNA catabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0043137 | biological_process | DNA replication, removal of RNA primer |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0003676 | molecular_function | nucleic acid binding |
| C | 0004518 | molecular_function | nuclease activity |
| C | 0004519 | molecular_function | endonuclease activity |
| C | 0004523 | molecular_function | RNA-DNA hybrid ribonuclease activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006401 | biological_process | RNA catabolic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0043137 | biological_process | DNA replication, removal of RNA primer |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0003676 | molecular_function | nucleic acid binding |
| D | 0004518 | molecular_function | nuclease activity |
| D | 0004519 | molecular_function | endonuclease activity |
| D | 0004523 | molecular_function | RNA-DNA hybrid ribonuclease activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0006401 | biological_process | RNA catabolic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0043137 | biological_process | DNA replication, removal of RNA primer |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MN A 1001 |
| Chain | Residue |
| A | ASP10 |
| A | ASP70 |
| A | HOH1037 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN B 1002 |
| Chain | Residue |
| B | ASP10 |
| B | GLY11 |
| B | HIS124 |
| B | HOH1003 |
| B | HOH1035 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN D 1003 |
| Chain | Residue |
| D | GLU48 |
| D | ASP70 |
| D | HOH1016 |
| D | HOH1018 |
| D | ASP10 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 564 |
| Details | Domain: {"description":"RNase H type-1","evidences":[{"source":"PROSITE-ProRule","id":"PRU00408","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 16 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"1311386","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 163 |
| Chain | Residue | Details |
| A | ASP10 | metal ligand |
| A | GLY11 | metal ligand |
| A | GLU48 | increase acidity, increase basicity, unknown |
| A | ASP70 | metal ligand |
| A | HIS124 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, unknown |
| A | ALA134 | increase acidity, metal ligand, proton acceptor, proton donor |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 163 |
| Chain | Residue | Details |
| B | ASP10 | metal ligand |
| B | GLY11 | metal ligand |
| B | GLU48 | increase acidity, increase basicity, unknown |
| B | ASP70 | metal ligand |
| B | HIS124 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, unknown |
| B | ALA134 | increase acidity, metal ligand, proton acceptor, proton donor |
| site_id | MCSA3 |
| Number of Residues | 6 |
| Details | M-CSA 163 |
| Chain | Residue | Details |
| C | ASP10 | metal ligand |
| C | GLY11 | metal ligand |
| C | GLU48 | increase acidity, increase basicity, unknown |
| C | ASP70 | metal ligand |
| C | HIS124 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, unknown |
| C | ALA134 | increase acidity, metal ligand, proton acceptor, proton donor |
| site_id | MCSA4 |
| Number of Residues | 6 |
| Details | M-CSA 163 |
| Chain | Residue | Details |
| D | ASP10 | metal ligand |
| D | GLY11 | metal ligand |
| D | GLU48 | increase acidity, increase basicity, unknown |
| D | ASP70 | metal ligand |
| D | HIS124 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, unknown |
| D | ALA134 | increase acidity, metal ligand, proton acceptor, proton donor |
